An In-Depth Understanding of Biomass Recalcitrance Using Natural Poplar Variants as the Feedstock.
Identifieur interne : 001529 ( Main/Exploration ); précédent : 001528; suivant : 001530An In-Depth Understanding of Biomass Recalcitrance Using Natural Poplar Variants as the Feedstock.
Auteurs : Xianzhi Meng [États-Unis] ; Yunqiao Pu [États-Unis] ; Chang Geun Yoo [États-Unis] ; Mi Li [États-Unis] ; Garima Bali [États-Unis] ; Doh-Yeon Park [États-Unis] ; Erica Gjersing [États-Unis] ; Mark F. Davis [États-Unis] ; Wellington Muchero [États-Unis] ; Gerald A. Tuskan [États-Unis] ; Timothy J. Tschaplinski [États-Unis] ; Arthur J. Ragauskas [États-Unis]Source :
- ChemSusChem [ 1864-564X ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Cellulose, Lignine, Populus.
- croissance et développement : Populus.
- Biocarburants, Biomasse, Biotechnologie, Hydrolyse, Polymérisation.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Cellulose, Lignin.
- chemical : Biofuels.
- chemistry : Populus.
- growth & development : Populus.
- Biomass, Biotechnology, Hydrolysis, Polymerization.
Abstract
In an effort to better understand the biomass recalcitrance, six natural poplar variants were selected as feedstocks based on previous sugar release analysis. Compositional analysis and physicochemical characterizations of these poplars were performed and the correlations between these physicochemical properties and enzymatic hydrolysis yield were investigated. Gel permeation chromatography (GPC) and 13 C solid state NMR were used to determine the degree of polymerization (DP) and crystallinity index (CrI) of cellulose, and the results along with the sugar release study indicated that cellulose DP likely played a more important role in enzymatic hydrolysis. Simons' stain revealed that the accessible surface area of substrate significantly varied among these variants from 17.3 to 33.2 mg g-1biomass as reflected by dye adsorption, and cellulose accessibility was shown as one of the major factors governing substrates digestibility. HSQC and 31 P NMR analysis detailed the structural features of poplar lignin variants. Overall, cellulose relevant factors appeared to have a stronger correlation with glucose release, if any, than lignin structural features. Lignin structural features, such as a phenolic hydroxyl group and the ratio of syringyl and guaiacyl (S/G), were found to have a more convincing impact on xylose release. Low lignin content, low cellulose DP, and high cellulose accessibility generally favor enzymatic hydrolysis; however, recalcitrance cannot be simply judged on any single substrate factor.
DOI: 10.1002/cssc.201601303
PubMed: 27882723
Affiliations:
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Bioproducts Institute, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renewable Bioproducts Institute, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332</wicri:regionArea><wicri:noRegion>30332</wicri:noRegion></affiliation></author><author><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Renewable Energy Laboratory, Golden, CO, 80401</wicri:regionArea><wicri:noRegion>80401</wicri:noRegion></affiliation></author><author><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Renewable Energy Laboratory, Golden, CO, 80401</wicri:regionArea><wicri:noRegion>80401</wicri:noRegion></affiliation></author><author><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation></author><author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. 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Tschaplinski</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation></author><author><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical & Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country 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wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001547</idno><idno type="wicri:Area/Main/Curation">001547</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001547</idno><idno type="wicri:Area/Main/Exploration">001547</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">An In-Depth Understanding of Biomass Recalcitrance Using Natural Poplar Variants as the Feedstock.</title><author><name sortKey="Meng, Xianzhi" sort="Meng, Xianzhi" uniqKey="Meng X" first="Xianzhi" last="Meng">Xianzhi Meng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical & Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author><author><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation></author><author><name sortKey="Yoo, Chang Geun" sort="Yoo, Chang Geun" uniqKey="Yoo C" first="Chang Geun" last="Yoo">Chang Geun Yoo</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation></author><author><name sortKey="Li, Mi" sort="Li, Mi" uniqKey="Li M" first="Mi" last="Li">Mi Li</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation></author><author><name sortKey="Bali, Garima" sort="Bali, Garima" uniqKey="Bali G" first="Garima" last="Bali">Garima Bali</name><affiliation wicri:level="1"><nlm:affiliation>Renewable Bioproducts Institute, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renewable Bioproducts Institute, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332</wicri:regionArea><wicri:noRegion>30332</wicri:noRegion></affiliation></author><author><name sortKey="Park, Doh Yeon" sort="Park, Doh Yeon" uniqKey="Park D" first="Doh-Yeon" last="Park">Doh-Yeon Park</name><affiliation wicri:level="1"><nlm:affiliation>Renewable Bioproducts Institute, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Renewable Bioproducts Institute, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332</wicri:regionArea><wicri:noRegion>30332</wicri:noRegion></affiliation></author><author><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Renewable Energy Laboratory, Golden, CO, 80401</wicri:regionArea><wicri:noRegion>80401</wicri:noRegion></affiliation></author><author><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Renewable Energy Laboratory, Golden, CO, 80401</wicri:regionArea><wicri:noRegion>80401</wicri:noRegion></affiliation></author><author><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation></author><author><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation></author><author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation></author><author><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical & Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831</wicri:regionArea><wicri:noRegion>37831</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Center for Renewable Carbon, Department of Forestry, Wildlife, and Fisheries, University Tennessee Institute of Agriculture, Knoxville, TN, 37996, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Renewable Carbon, Department of Forestry, Wildlife, and Fisheries, University Tennessee Institute of Agriculture, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author></analytic><series><title level="j">ChemSusChem</title><idno type="eISSN">1864-564X</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biofuels (MeSH)</term><term>Biomass (MeSH)</term><term>Biotechnology (MeSH)</term><term>Cellulose (chemistry)</term><term>Hydrolysis (MeSH)</term><term>Lignin (chemistry)</term><term>Polymerization (MeSH)</term><term>Populus (chemistry)</term><term>Populus (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biocarburants (MeSH)</term><term>Biomasse (MeSH)</term><term>Biotechnologie (MeSH)</term><term>Cellulose (composition chimique)</term><term>Hydrolyse (MeSH)</term><term>Lignine (composition chimique)</term><term>Polymérisation (MeSH)</term><term>Populus (composition chimique)</term><term>Populus (croissance et développement)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulose</term><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biofuels</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellulose</term><term>Lignine</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Biotechnology</term><term>Hydrolysis</term><term>Polymerization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biocarburants</term><term>Biomasse</term><term>Biotechnologie</term><term>Hydrolyse</term><term>Polymérisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In an effort to better understand the biomass recalcitrance, six natural poplar variants were selected as feedstocks based on previous sugar release analysis. Compositional analysis and physicochemical characterizations of these poplars were performed and the correlations between these physicochemical properties and enzymatic hydrolysis yield were investigated. Gel permeation chromatography (GPC) and <sup>13</sup> C solid state NMR were used to determine the degree of polymerization (DP) and crystallinity index (CrI) of cellulose, and the results along with the sugar release study indicated that cellulose DP likely played a more important role in enzymatic hydrolysis. Simons' stain revealed that the accessible surface area of substrate significantly varied among these variants from 17.3 to 33.2 mg g-1biomass as reflected by dye adsorption, and cellulose accessibility was shown as one of the major factors governing substrates digestibility. HSQC and <sup>31</sup> P NMR analysis detailed the structural features of poplar lignin variants. Overall, cellulose relevant factors appeared to have a stronger correlation with glucose release, if any, than lignin structural features. Lignin structural features, such as a phenolic hydroxyl group and the ratio of syringyl and guaiacyl (S/G), were found to have a more convincing impact on xylose release. Low lignin content, low cellulose DP, and high cellulose accessibility generally favor enzymatic hydrolysis; however, recalcitrance cannot be simply judged on any single substrate factor.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27882723</PMID><DateCompleted><Year>2017</Year><Month>05</Month><Day>23</Day></DateCompleted><DateRevised><Year>2017</Year><Month>05</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1864-564X</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>2017</Year><Month>Jan</Month><Day>10</Day></PubDate></JournalIssue><Title>ChemSusChem</Title><ISOAbbreviation>ChemSusChem</ISOAbbreviation></Journal><ArticleTitle>An In-Depth Understanding of Biomass Recalcitrance Using Natural Poplar Variants as the Feedstock.</ArticleTitle><Pagination><MedlinePgn>139-150</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/cssc.201601303</ELocationID><Abstract><AbstractText>In an effort to better understand the biomass recalcitrance, six natural poplar variants were selected as feedstocks based on previous sugar release analysis. Compositional analysis and physicochemical characterizations of these poplars were performed and the correlations between these physicochemical properties and enzymatic hydrolysis yield were investigated. Gel permeation chromatography (GPC) and <sup>13</sup> C solid state NMR were used to determine the degree of polymerization (DP) and crystallinity index (CrI) of cellulose, and the results along with the sugar release study indicated that cellulose DP likely played a more important role in enzymatic hydrolysis. Simons' stain revealed that the accessible surface area of substrate significantly varied among these variants from 17.3 to 33.2 mg g-1biomass as reflected by dye adsorption, and cellulose accessibility was shown as one of the major factors governing substrates digestibility. HSQC and <sup>31</sup> P NMR analysis detailed the structural features of poplar lignin variants. Overall, cellulose relevant factors appeared to have a stronger correlation with glucose release, if any, than lignin structural features. Lignin structural features, such as a phenolic hydroxyl group and the ratio of syringyl and guaiacyl (S/G), were found to have a more convincing impact on xylose release. Low lignin content, low cellulose DP, and high cellulose accessibility generally favor enzymatic hydrolysis; however, recalcitrance cannot be simply judged on any single substrate factor.</AbstractText><CopyrightInformation>© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>Xianzhi</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pu</LastName><ForeName>Yunqiao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yoo</LastName><ForeName>Chang Geun</ForeName><Initials>CG</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Mi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bali</LastName><ForeName>Garima</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Renewable Bioproducts Institute, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Doh-Yeon</ForeName><Initials>DY</Initials><AffiliationInfo><Affiliation>Renewable Bioproducts Institute, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gjersing</LastName><ForeName>Erica</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davis</LastName><ForeName>Mark F</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Renewable Energy Laboratory, Golden, CO, 80401, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muchero</LastName><ForeName>Wellington</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tuskan</LastName><ForeName>Gerald A</ForeName><Initials>GA</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tschaplinski</LastName><ForeName>Timothy J</ForeName><Initials>TJ</Initials><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ragauskas</LastName><ForeName>Arthur J</ForeName><Initials>AJ</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1420-6678</Identifier><AffiliationInfo><Affiliation>Department of Chemical & Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Renewable Carbon, Department of Forestry, Wildlife, and Fisheries, University Tennessee Institute of Agriculture, Knoxville, TN, 37996, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>12</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>ChemSusChem</MedlineTA><NlmUniqueID>101319536</NlmUniqueID><ISSNLinking>1864-5631</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D056804">Biofuels</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D056804" MajorTopicYN="Y">Biofuels</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="Y">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058105" MajorTopicYN="N">Polymerization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">biomass recalcitrance</Keyword><Keyword MajorTopicYN="Y">cellulose</Keyword><Keyword MajorTopicYN="Y">crystallinity</Keyword><Keyword MajorTopicYN="Y">degree of polymerization</Keyword><Keyword MajorTopicYN="Y">lignin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>09</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>10</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>11</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>5</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>11</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27882723</ArticleId><ArticleId IdType="doi">10.1002/cssc.201601303</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Meng, Xianzhi" sort="Meng, Xianzhi" uniqKey="Meng X" first="Xianzhi" last="Meng">Xianzhi Meng</name></noRegion><name sortKey="Bali, Garima" sort="Bali, Garima" uniqKey="Bali G" first="Garima" last="Bali">Garima Bali</name><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><name sortKey="Davis, Mark F" sort="Davis, Mark F" uniqKey="Davis M" first="Mark F" last="Davis">Mark F. Davis</name><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name><name sortKey="Gjersing, Erica" sort="Gjersing, Erica" uniqKey="Gjersing E" first="Erica" last="Gjersing">Erica Gjersing</name><name sortKey="Li, Mi" sort="Li, Mi" uniqKey="Li M" first="Mi" last="Li">Mi Li</name><name sortKey="Li, Mi" sort="Li, Mi" uniqKey="Li M" first="Mi" last="Li">Mi Li</name><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name><name sortKey="Muchero, Wellington" sort="Muchero, Wellington" uniqKey="Muchero W" first="Wellington" last="Muchero">Wellington Muchero</name><name sortKey="Park, Doh Yeon" sort="Park, Doh Yeon" uniqKey="Park D" first="Doh-Yeon" last="Park">Doh-Yeon Park</name><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name><name sortKey="Tuskan, Gerald A" sort="Tuskan, Gerald A" uniqKey="Tuskan G" first="Gerald A" last="Tuskan">Gerald A. Tuskan</name><name sortKey="Yoo, Chang Geun" sort="Yoo, Chang Geun" uniqKey="Yoo C" first="Chang Geun" last="Yoo">Chang Geun Yoo</name><name sortKey="Yoo, Chang Geun" sort="Yoo, Chang Geun" uniqKey="Yoo C" first="Chang Geun" last="Yoo">Chang Geun Yoo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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